Publications related to fish diseases
Cascarano, M.C., Ruetten, M., Vaughan, L., Tsertou, M.I., Georgopoulou, D., Keklikoglou, K., Papandroulakis, N. and Katharios, P., 2022. Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings. Microorganisms, 10(3), p.627
Cascarano, M.C., Stavrakidis-Zachou, O., Mladineo, I., Thompson, K.D., Papandroulakis, N. and Katharios, P., 2021. Mediterranean Aquaculture in a Changing Climate: Temperature Effects on Pathogens and Diseases of Three Farmed Fish Species. Pathogens, 10(9), p.1205.
Smyrli, M., Anka, I.Z., Koutsoni, O., Dotsika, E., Kyriazis, I.D., Pavlidis, M. and Katharios, P., 2022. Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment. Fish & Shellfish Immunology, 123, pp.381-387.
Tsertou, M. I., Smyrli, M., Kokkari, C., Antonopoulou, E., & Katharios, P. (2018). The aetiology of systemic granulomatosis in meagre (Argyrosomus regius): The “Nocardia” hypothesis. Aquaculture Reports, 12, 5-11.
Smyrli, M., Prapas, A., Rigos, G., Kokkari, C., Arfara, S., Papalexiou, E., Katharios, P. Aeromonas veronii infection associated with morbidity and mortality in farmed European seabass (Dicentrarchus labrax). Fish Pathology Vol, 52, No.2, 68-81
Andree, K.B., Roque, A., Duncan, Ν., Gisbert, Ε., Estevez, Α., Tsertou, Μ.Ι., Katharios, P. 2015. Diplectanum sciaenae (Van Beneden & Hesse, 1863) (Monogenea) infecting meagre, Argyrosomus regius (Asso, 1801) broodstock in Catalonia, Spain. A case report, Veterinary Parasitology: Regional Studies and Reports 1-2, 75-79, Available online 26 February 2016, ISSN 2405-9390, http://dx.doi.org/10.1016/j.vprsr.2016.02.006.
Seth-Smith, H.B., Dourala, N., Fehr, A., Katharios, P., Qi, W., Ruetten, M., Mateos, J.M., Nufer, L., Weilenmann. R., Ziegler, U., Thomson, N.R., N.R., Schlapbach R., Vaughan, L. 2015. Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria. ISME J, 10, 1791-1803; doi:10.1038/ismej.2015.223
Katharios, P., Seth-Smith, H.B., Fehr, A., Mateos, J.M., Qi, W., Richter, D., Nufer, L., Ruetten, M., Guevara Soto, M., Ziegler, U., Thomson, N.R., Schlapbach R., Vaughan, L. 2015. Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: striking genomic and morphological features of novel Endozoicomonas sp. Scientific Reports 5, 17609, doi:10.1038/srep17609
Kalatzis, P., Kokkari, C., Katharios P. 2015. Morphological characterisation and phylogenetic relationships of Zschokkella candia n. sp. from the gall-bladder of Sparisoma cretense (L.) (Perciformes: Scaridae) in the Sea of Crete off Greece. Systematic Parasitology 92(3), 211-218.
Katharios, P., Kokkari, C., Dourala, N., Smyrli, M., 2015. First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean. BMC Vet. Res. 11, 155. doi:10.1186/s12917-015-0482-x
Katharios, P., Kokkari, C., Sterioti, A., Smyrli, M., Kalatzis, P. 2014. Enteromyxum leei infection in parrotfish, Sparisoma cretense: Histopathological, morphological and molecular study. Veterinary Parasitology. 199. 136-143
Rigos, G., Karagouni, E., Kyriazis, I., Athanasiou, E., Grigorakis, K., Kotou, E., Katharios, P. 2013. In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans. Aquaculture. 416-417. 185-191
Kalatzis, P.G., Kokkari, C., Katharios, P. 2013. Description and relationships of two novel species of Ceratomyxa Thelohan, 1892 infecting the gallbladders of Aulopiformes: Atlantic lizardfish Synodus saurus Linnaeus, 1758 and royal flagfin Aulopus filamentosus Bloch, 1792 from Cretan Sea, Greece. Parasitology Research. 112. 2055-2061
Katharios, P., Papadaki, M., Ternengo, S., Kantham, P.K., Zeri, C., Petraki, P.E., Divanach, P. 2011. Chronic ulcerative dermatopathy in cultured marine fishes. Comparative study in sharpsnout sea bream, Diplodus puntazzo (Walbaum). Journal of Fish Diseases. 34. 459-474
Katharios, P., Rigos, G., Divanach, P. 2011. Enteromyxum leei (Myxozoa), a Lethal Intruder of Tropical Pet Fish: First Case in Humphead Wrasse, Cheilinus undulatus (Rüppell, 1835). Journal of Exotic Pet Medicine. 20. 138-143
Katharios, P., Tsigenopoulos, C.S. 2010. First report of nodavirus outbreak in cultured juvenile shi drum, Umbrina cirrosa L., in Greece. Aquaculture Research. 42. 147-152
Rigos, G., Katharios, P. 2010. Pathological obstacles of newly-introduced fish species in Mediterranean mariculture: A review. Reviews in Fish Biology and Fisheries. 20. 47-70
Cascarano, M.C., Stavrakidis-Zachou, O., Mladineo, I., Thompson, K.D., Papandroulakis, N. and Katharios, P., 2021. Mediterranean Aquaculture in a Changing Climate: Temperature Effects on Pathogens and Diseases of Three Farmed Fish Species. Pathogens, 10(9), p.1205.
Smyrli, M., Anka, I.Z., Koutsoni, O., Dotsika, E., Kyriazis, I.D., Pavlidis, M. and Katharios, P., 2022. Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment. Fish & Shellfish Immunology, 123, pp.381-387.
Tsertou, M. I., Smyrli, M., Kokkari, C., Antonopoulou, E., & Katharios, P. (2018). The aetiology of systemic granulomatosis in meagre (Argyrosomus regius): The “Nocardia” hypothesis. Aquaculture Reports, 12, 5-11.
Smyrli, M., Prapas, A., Rigos, G., Kokkari, C., Arfara, S., Papalexiou, E., Katharios, P. Aeromonas veronii infection associated with morbidity and mortality in farmed European seabass (Dicentrarchus labrax). Fish Pathology Vol, 52, No.2, 68-81
Andree, K.B., Roque, A., Duncan, Ν., Gisbert, Ε., Estevez, Α., Tsertou, Μ.Ι., Katharios, P. 2015. Diplectanum sciaenae (Van Beneden & Hesse, 1863) (Monogenea) infecting meagre, Argyrosomus regius (Asso, 1801) broodstock in Catalonia, Spain. A case report, Veterinary Parasitology: Regional Studies and Reports 1-2, 75-79, Available online 26 February 2016, ISSN 2405-9390, http://dx.doi.org/10.1016/j.vprsr.2016.02.006.
Seth-Smith, H.B., Dourala, N., Fehr, A., Katharios, P., Qi, W., Ruetten, M., Mateos, J.M., Nufer, L., Weilenmann. R., Ziegler, U., Thomson, N.R., N.R., Schlapbach R., Vaughan, L. 2015. Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria. ISME J, 10, 1791-1803; doi:10.1038/ismej.2015.223
Katharios, P., Seth-Smith, H.B., Fehr, A., Mateos, J.M., Qi, W., Richter, D., Nufer, L., Ruetten, M., Guevara Soto, M., Ziegler, U., Thomson, N.R., Schlapbach R., Vaughan, L. 2015. Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: striking genomic and morphological features of novel Endozoicomonas sp. Scientific Reports 5, 17609, doi:10.1038/srep17609
Kalatzis, P., Kokkari, C., Katharios P. 2015. Morphological characterisation and phylogenetic relationships of Zschokkella candia n. sp. from the gall-bladder of Sparisoma cretense (L.) (Perciformes: Scaridae) in the Sea of Crete off Greece. Systematic Parasitology 92(3), 211-218.
Katharios, P., Kokkari, C., Dourala, N., Smyrli, M., 2015. First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean. BMC Vet. Res. 11, 155. doi:10.1186/s12917-015-0482-x
Katharios, P., Kokkari, C., Sterioti, A., Smyrli, M., Kalatzis, P. 2014. Enteromyxum leei infection in parrotfish, Sparisoma cretense: Histopathological, morphological and molecular study. Veterinary Parasitology. 199. 136-143
Rigos, G., Karagouni, E., Kyriazis, I., Athanasiou, E., Grigorakis, K., Kotou, E., Katharios, P. 2013. In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans. Aquaculture. 416-417. 185-191
Kalatzis, P.G., Kokkari, C., Katharios, P. 2013. Description and relationships of two novel species of Ceratomyxa Thelohan, 1892 infecting the gallbladders of Aulopiformes: Atlantic lizardfish Synodus saurus Linnaeus, 1758 and royal flagfin Aulopus filamentosus Bloch, 1792 from Cretan Sea, Greece. Parasitology Research. 112. 2055-2061
Katharios, P., Papadaki, M., Ternengo, S., Kantham, P.K., Zeri, C., Petraki, P.E., Divanach, P. 2011. Chronic ulcerative dermatopathy in cultured marine fishes. Comparative study in sharpsnout sea bream, Diplodus puntazzo (Walbaum). Journal of Fish Diseases. 34. 459-474
Katharios, P., Rigos, G., Divanach, P. 2011. Enteromyxum leei (Myxozoa), a Lethal Intruder of Tropical Pet Fish: First Case in Humphead Wrasse, Cheilinus undulatus (Rüppell, 1835). Journal of Exotic Pet Medicine. 20. 138-143
Katharios, P., Tsigenopoulos, C.S. 2010. First report of nodavirus outbreak in cultured juvenile shi drum, Umbrina cirrosa L., in Greece. Aquaculture Research. 42. 147-152
Rigos, G., Katharios, P. 2010. Pathological obstacles of newly-introduced fish species in Mediterranean mariculture: A review. Reviews in Fish Biology and Fisheries. 20. 47-70
Publications related to Genomics
Katharios, P., Kalatzis, P.G., Kokkari, P., Pavlidis, M., Wang, Q. 2019. Edwardsiella anguillarum, isolated from Greek aquaculture containing an inducible prophage; genomic characterization and virulence. Frontiers in Microbiology 10, 141.
Qi, W., Cascarano, MC, Schlapbach R., Katharios, P., Vaughan, L., Seth-Smith, HMB. Ca. Endozoicomonas cretensis: a novel fish pathogen characterised by genome plasticity. Genome Biology and Evolution 12, 5–11. doi:10.1016/j.aqrep.2018.08.002
Katharios, P. Virulence regulation during late infection by a fish pathogen; sense and sensibility of bacteria may lead to novel vaccine development strategy. Virulence. doi: 10.1080/21505594.2017.1344807
Vaughan, L., Seth-Smith, H., Katharios, P., Dourala, P., Mateos, J., Fehr, A., Nufer, L., Ruetten, M., Guevara, M. Ca. Similichlamydia in epitheliocystis co-infection of gilthead seabream gills: unique morphological features of a deep branching chlamydial family. Frontiers in Microbiology. vol 8, article 508
Qi, W., Vaughan, L., Katharios, P., Schlapbach, R., Seth-Smith, H. 2016. Host-associated genomic features of the novel uncultured intracellular pathogen Ca. Ichthyocystis revealed by direct sequencing of epitheliocysts. Genome Biology and Evolution 8(6):1672–1689. doi:10.1093/gbe/evw111
Castillo, D., D’Alvise, P., Kalatzis, P.G., Kokkari, C., Middelboe, M., Gram, L., Liu, S., Katharios, P., 2015a. Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens. Genome Announc. 3, e00729–15.
Castillo, D., D’Alvise, P., Middelboe, M., Gram, L., Liu, S., Kalatzis, P.G., Kokkari, C., Katharios, P., 2015b. Draft genome sequences of the fish pathogen Vibrio harveyi strains VH2 and VH5. Genome Announc. 3, e01062–15.
Castillo, D., Jun, J.W., D’Alvise, P., Middelboe, M., Gram, L., Liu, S., Katharios, P., 2015c. Draft genome sequence of Vibrio parahaemolyticus VH3, isolated from an aquaculture environment in Greece. Genome Announc. 3, e00731–15.
Qi, W., Cascarano, MC, Schlapbach R., Katharios, P., Vaughan, L., Seth-Smith, HMB. Ca. Endozoicomonas cretensis: a novel fish pathogen characterised by genome plasticity. Genome Biology and Evolution 12, 5–11. doi:10.1016/j.aqrep.2018.08.002
Katharios, P. Virulence regulation during late infection by a fish pathogen; sense and sensibility of bacteria may lead to novel vaccine development strategy. Virulence. doi: 10.1080/21505594.2017.1344807
Vaughan, L., Seth-Smith, H., Katharios, P., Dourala, P., Mateos, J., Fehr, A., Nufer, L., Ruetten, M., Guevara, M. Ca. Similichlamydia in epitheliocystis co-infection of gilthead seabream gills: unique morphological features of a deep branching chlamydial family. Frontiers in Microbiology. vol 8, article 508
Qi, W., Vaughan, L., Katharios, P., Schlapbach, R., Seth-Smith, H. 2016. Host-associated genomic features of the novel uncultured intracellular pathogen Ca. Ichthyocystis revealed by direct sequencing of epitheliocysts. Genome Biology and Evolution 8(6):1672–1689. doi:10.1093/gbe/evw111
Castillo, D., D’Alvise, P., Kalatzis, P.G., Kokkari, C., Middelboe, M., Gram, L., Liu, S., Katharios, P., 2015a. Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens. Genome Announc. 3, e00729–15.
Castillo, D., D’Alvise, P., Middelboe, M., Gram, L., Liu, S., Kalatzis, P.G., Kokkari, C., Katharios, P., 2015b. Draft genome sequences of the fish pathogen Vibrio harveyi strains VH2 and VH5. Genome Announc. 3, e01062–15.
Castillo, D., Jun, J.W., D’Alvise, P., Middelboe, M., Gram, L., Liu, S., Katharios, P., 2015c. Draft genome sequence of Vibrio parahaemolyticus VH3, isolated from an aquaculture environment in Greece. Genome Announc. 3, e00731–15.
Publications related to Phage Therapy
Droubogiannis, S.; Pavlidi, L.; Tsertou, M.I.; Kokkari, C.; Skliros, D.; Flemetakis, E.; Katharios, P. Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus. Pathogens 2022, 11, 848. https://doi.org/10.3390/pathogens11080848
Droubogiannis S, Katharios P. 2022. Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi. Pathogens 11(6):630. https://doi.org/10.3390/pathogens11060630Misol, G.N., Jr.; Kokkari, C.; Katharios, P. 2020. Biological and Genomic Characterization of a Novel Jumbo Bacteriophage, vB_VhaM_pir03 with Broad Host Lytic Activity against Vibrio harveyi. Pathogens 9, 1051.
Kalatzis PG, Carstens AB, Katharios P, Castillo D, Hansen LH, Middelboe M. Complete Genome Sequence of Vibrio anguillarum Nontailed Bacteriophage NO16. Microbiol Resour Announc. 2019 Apr 11;8(15):e00020-19.
León M, Kokkari C, García K, Castillo D, Katharios P, Bastías R. Diversification of Vibrio anguillarum driven by the bacteriophage CHOED. Frontiers in Microbiology. 2019;10:1396.
Kalatzis, P. G., Castillo, D., Katharios, P., Middelboe M. 2018. Bacteriophage Interactions with Marine Pathogenic Vibrios: Implications for Phage Therapy. Antibiotics 7(1), 15; doi:10.3390/antibiotics7010015.
Kokkari, C., Sarropoulou, E., Bastias, R., Mandalakis, M., Katharios, P. 2018. Isolation and characterization of a novel bacteriophage infecting Vibrio alginolyticus. Archives of Microbiology. https://doi.org/10.1007/s00203-018-1480-8
Katharios, P., Kalatzis, PG, Kokkari, C., Sarropoulou, E., and Middelboe, M. 2017. Isolation and characterization of an N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves. PLoS ONE 12(12): e0190083. https://doi.org/10.1371/journal.pone.0190083
Kalatzis, P.G., Rørbo, N., Castillo, D., Mauritzen, J.J., Jørgensen, J., Kokkari, C., Zhang, F., Katharios, P., Middelboe, M. Stumbling across the same phage: Comparative genomics of widespread temperate phages infecting the fish pathogen Vibrio anguillarum (2017) Viruses, 9 (5), art. no. 122, doi: 10.3390/v9050122
. https://doi.org/10.3389/fmicb.2017.00508
Skliros, D., Kalatzis, P., Katharios, P., Flemetakis, E. 2016. Comparative functional genomic analysis of two vibrio phages reveals complex metabolic interactions with the host cell. Frontiers in Microbiology, vol 7, article 1807. doi: 10.3389/fmicb.2016.01807
Kalatzis, P.G, Bastías, R., Kokkari, C., Katharios, P. 2016. Isolation and Characterization of Two Lytic Bacteriophages, φSt2 and φGrn1; Phage Therapy Application for Biological Control of Vibrio alginolyticus in Aquaculture Live Feeds. PLoS ONE 11(3): e0151101. doi: 10.1371/journal.pone.0151101
Droubogiannis S, Katharios P. 2022. Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi. Pathogens 11(6):630. https://doi.org/10.3390/pathogens11060630Misol, G.N., Jr.; Kokkari, C.; Katharios, P. 2020. Biological and Genomic Characterization of a Novel Jumbo Bacteriophage, vB_VhaM_pir03 with Broad Host Lytic Activity against Vibrio harveyi. Pathogens 9, 1051.
Kalatzis PG, Carstens AB, Katharios P, Castillo D, Hansen LH, Middelboe M. Complete Genome Sequence of Vibrio anguillarum Nontailed Bacteriophage NO16. Microbiol Resour Announc. 2019 Apr 11;8(15):e00020-19.
León M, Kokkari C, García K, Castillo D, Katharios P, Bastías R. Diversification of Vibrio anguillarum driven by the bacteriophage CHOED. Frontiers in Microbiology. 2019;10:1396.
Kalatzis, P. G., Castillo, D., Katharios, P., Middelboe M. 2018. Bacteriophage Interactions with Marine Pathogenic Vibrios: Implications for Phage Therapy. Antibiotics 7(1), 15; doi:10.3390/antibiotics7010015.
Kokkari, C., Sarropoulou, E., Bastias, R., Mandalakis, M., Katharios, P. 2018. Isolation and characterization of a novel bacteriophage infecting Vibrio alginolyticus. Archives of Microbiology. https://doi.org/10.1007/s00203-018-1480-8
Katharios, P., Kalatzis, PG, Kokkari, C., Sarropoulou, E., and Middelboe, M. 2017. Isolation and characterization of an N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves. PLoS ONE 12(12): e0190083. https://doi.org/10.1371/journal.pone.0190083
Kalatzis, P.G., Rørbo, N., Castillo, D., Mauritzen, J.J., Jørgensen, J., Kokkari, C., Zhang, F., Katharios, P., Middelboe, M. Stumbling across the same phage: Comparative genomics of widespread temperate phages infecting the fish pathogen Vibrio anguillarum (2017) Viruses, 9 (5), art. no. 122, doi: 10.3390/v9050122
. https://doi.org/10.3389/fmicb.2017.00508
Skliros, D., Kalatzis, P., Katharios, P., Flemetakis, E. 2016. Comparative functional genomic analysis of two vibrio phages reveals complex metabolic interactions with the host cell. Frontiers in Microbiology, vol 7, article 1807. doi: 10.3389/fmicb.2016.01807
Kalatzis, P.G, Bastías, R., Kokkari, C., Katharios, P. 2016. Isolation and Characterization of Two Lytic Bacteriophages, φSt2 and φGrn1; Phage Therapy Application for Biological Control of Vibrio alginolyticus in Aquaculture Live Feeds. PLoS ONE 11(3): e0151101. doi: 10.1371/journal.pone.0151101
